Science Notebook

Vaccine Views 

Video #1
Part 1:
For this video, fill in FUN FACTS about how the vaccines and how they work:
Polio Live Vaccine
Similarities
Between Both
Polio Dead Vaccine
1.Liquid, drink it
2. Humoral & cell-mediated
3. 95% affected
4.passes person to person
5.
1.Humoral
2. High effective rates
3.
4.
5.
1.can not replicate, live
2.injected in muscle
3.99% affective
4.can not pass on the immunity
5.

Part 2:
What was the purpose of this video? How does the producer of the video use rhetoric to support their purpose? Give three pieces of evidence from the video to support your claim. (Example of evidence can be narrator’s tone, the information presented, information not presented, music played, and/or images shown.)
The purpose of this video is to show the differences and similarities between two vaccines polio live and polio dead. The narrator would use rhetoric to support the purpose by going back and forth between each vaccine. He was comparing them and drawing pictures along with it to make it more understandable.


  1. What diseases do vaccines prevent?
Vaccines prevent whooping cough, chickenpox and meningitis.
  1. What is herd immunity?
Herd immunity is the immunity or resistance to a particular infection that occurs in a group of people or animals when a very high percentage of individuals have been vaccinated or previously exposed to the infection.
  1. What does it mean to be immunocompromised?
It means to have impaired immune system.
  1. What kind of people would be considered immunocompromised?
They are considered immunocompromised when the vaccines do not help their body.

  1. How does herd immunity help people who are immunocompromised?
It helps them  their body from getting infected.
  1. What does it mean to “exempt vaccines”?
To not be vaccinated
  1. What two ways can parents “exempt vaccines”?
  • Making it their choice
Part 2:
What was the purpose of this video? How does the producer of the video use rhetoric to support their purpose? Give three pieces of evidence from the video to support your claim. (Example of evidence can be narrator’s tone, the information presented, information not presented, music played, and/or images shown.)
The purpose of the video is to inform us about vaccines and how they should make everyone get them before they start school. The narrator uses videos such as public speakers, shows pictures of kids that have had a disease and shows the a speech from rhett and his parents.

Video #3 (For today, just watch the first 16 minutes of this video to complete the activity)

Part 1:
  1. What is the theory behind vaccines?
People or doctors are pro vaccine or anti vaccine is the theory.
  1. What is the stigma around a person who questions vaccines?
The stigma of a person who questions vaccines it does not make them anti vaccine.
  1. What is “informed consent”?
It is permission already having the knowledge of knowing risks.
  1. Do doctors give “informed consent” to patients on vaccines?
Doctors don’t really have “informed consent”.
  1. Who controls vaccine laws?
The state makes the vaccine laws.
  1. What is California’s vaccine law? (Not mentioned in video, please Google it.)
  1. How much knowledge do doctors receive about vaccines in medical school?
They are not really taught about vaccines only handed a paper with the instructions.
Part 2:
What was the purpose of this video? How does the producer of the video use rhetoric to support their purpose? Give three pieces of evidence from the video to support your claim. (Example of evidence can be narrator’s tone, the information presented, information not presented, music played, and/or images shown.)
The purpose of the video is informing us about vaccine laws and how doctors know hot to use them. It made it a stronger video when they were interviewing actual doctors. This video was very interesting out of all the videos.

Summary Questions:

  1. What is the “vaccine war”?
The vaccine war is the controversial idea of whether or not vaccinations should be optional.
  1. How has media shaped the “vaccine war”?
The media is holding all the information about vaccines and informing people about them.
  1. How has researching vaccine ingredients, learning about the immune system, and watching three videos with separate agendas helped you?
It gives me more of understanding about vaccines and how it can affect us in a good or bad way.

Blood typing 



Trial Number
Reaction with Anti-A
Reaction with Anti-B
Reaction with Anti-Rh
Determined blood type
1
yes
no
yes
A positive

  • What blood type is needed for a transfusion for a Type O positive patient?
Type O positive is necessary for someone who needs a Type O transfusion

  • What would happen if someone was transfused with an incompatible blood type?
If someone was transfused with an incompatible blood type then they would have
a reaction.


How Are Antibodies Used for Blood Typing?-Lab Report

Introduction: In NEW school we are currently learning about immunology and how are body reacts to certain vaccines. In this lab we are blood typing to see what type blood we are and to learn about how are immune system works
Purpose: What blood type is Kelly going to be?
Hypothesis: If kellys blood is B negative, then antigen b will clump up , because
there will be a reaction to the antigen b and only that liquid.

Materials:
  1. Blood typing slide (4)
  2. Antisera A and B
  3. Anti-Rh factor
  4. Mixing sticks
  5. Synthetic blood samples (4); Note: These do not contain real blood and thus are completely safe to handle. They will however accurately simulate the blood typing process.
  6. Disposable plastic pipettes (4); these are for moving the blood samples to the blood typing slide
  7. Disposable gloves
  8. Bleach bath
  9. Cotton balls
  10. Lancets

Procedure:
  1. In your lab notebook make a data table, to record all your data and observations.
  2. Using a disposable toothpick, place a drop of blood sample in each well of a blood typing slide.
  3. Add a drop of synthetic anti-A to the well labeled A.
  4. Add a drop of synthetic anti-B serum to the well labeled B.
  5. Add a drop of synthetic anti-Rh serum to the well labeled Rh.
  6. Using a different color mixing stick for each well, gently stir the blood and antiserum drops for 30 seconds. Remember to discard each mixing stick after a single use to avoid contamination to your samples.
  7. After 30 seconds of stirring carefully examine the liquid in the wells.
    1. If the liquid is clear or light pink with no particles or cloudiness formed, then no reaction has occurred and you should mark "No" in the appropriate box in the data table.
    2. If there are solid particles that have formed in mixing the sample or antiserum (they may be darker or lighter than the original liquid), then a reaction has occurred and you should mark "Yes" in the appropriate box. Also, if the liquid has very small particles formed, giving a cloudy appearance to the liquid, then a reaction has occurred and you should mark "Yes" in the appropriate box.
    3. It is important to look very closely at the wells and only do one well at a time. Be sure to stir at least 30 seconds with the plastic stick. The final product may be clear, white or dark pink depending on the combination of blood sample and antiserum, so look carefully to see if small or large particles have formed in the well.
    4. Reference the photos in Figure 1 for help determining if a reaction has occurred or not. If you continue to have trouble read the FAQ for this project.
  8. Record the results of your blood sample in the data table
  9. Place the toothpicks, blood typing slide, and cotton balls into the bleach bath
  10. Compare your results with the data table below:


Source: “How Are Antibodies Used for Blood Typing?” By Science Buddies



Data Table:

Trial Number
Reaction with Anti-A
Reaction with Anti-B
Reaction with Anti-Rh
Determined blood type
1
yes
no
yes
A positive


Conclusion: This section shows YOUR UNDERSTANDING of the lab. A strong conclusion will be 2 paragraphs long and follow the argumentation from evidence format.
Paragraph One:
  • Claim –Kellys blood type was B-.
  • Evidence – Your evidence is the data you collected during the lab. Use your data to support and backup your claim. Discuss the patterns in your data; reference the ACTUAL numbers and trends in your data table and graph.
  • Explain – Provide an explanation for what your data tells you. Explain why or how the evidence/data supports your claim.
Paragraph Two:
  • Reflect on your original hypothesis, does your data support your original idea or not? Explain your answer. (Paper that earns a 3 or 4 will do this well).
  • The errors that could have happened would be putting the wrong liquid in the wrong holder place on the tray. As well as not sanitizing good.



Role of phagocytes in innate or nonspecific community 


What is the point of the immune system?
it is to keep out shady things from the body that can do damage to the body. (bacteria, bad proteins,viruses .. etc)


What is the difference between the first line of defense and second line of defense?

The first line of defense is to keep things out and more obvious our skin and oils.

What is innate immunity mean?

It means that they generally respond to the things that came bad.

What is non-specific immunity mean?

The initial immune response reaction against foreign antigens.
What are three examples of first line of defense?
Bacteria, bad proteins, viruses
What are the two examples of the second line of defense?
inflammatory response and phagocytes
What is a pathogen?
A bacteria or virus that can cause a disease

What is a phagocyte?
A class of cell that can eat up pathogens.
What is major histocompatibility complex II (MHC II)?
After they the foreign cells and take their proteins and flag

What is an antigen?
A toxin or foreign substance that induces an immune response

What is a virus? How do viruses work?

What is a virus?
-not living, very tiny ,complex collection of organic matter

What is the structure of a virus? (The anatomy of a virus)

-A strand of genetic info

How do viruses work?

-It must infect some kind of cells so it will be able to reproduce genetic material

-viruses are able to infect healthy cells and convert them into mindless virus producing robots.

How can a virus enter the body?
-Eat a certain type of food, water, swimming
What is bigger a virus or bacteria?
bacteria
How do viruses replicate?
-viruses are able to infect healthy cells and convert them into mindless virus producing robots.
-When in the cell the virus will then begin to use its molecular arsenal to hijack the cells protein manufacturing systems and DNA replication mechanism after the virus has integrated itself into the cells own DNA the cell will then replicate.

Vaccine

Questions-

How much is toxic?
How many people experience side effects?

Polio
9 ingredients 6 of those are toxic
formaldehyde, streptomycin and polymyxin B are toxic in high quantities

DTap
15 ingredients
10 ingredients toxic

MMR-
13 ingredients
measels, mumps and rubella
None are toxic
limited info in chick embryo cell culture and WI-38 human diploid lung

HIB (Haemophilus influenzae type B)
12 different ingredients
toxic if allergic to milk based proteins modified mueller and miller medium could be potentially dangerous

Hepatitis B
5 ingredients - 1st brand
9 ingredients - second brand
not toxic- phosphate buffer, yeast protein, dextrose,mineral salts
toxic- formaldehyde, sodium chloride, potassium aluminum sulfate 

Hepatitis A 
16 ingredients
 not toxic- formalin, human diploid cells 
toxic- sodium chloride, sodium borate, neomycin

HPV
10 ingredients 
non toxic- carbohydrates, vitamins 
protecting genitals from cancer 
limited info- mineral salts 


Science Assessment #7 Revamp

Independent vs Dependent variables

Where does our water come from?

Teach backs



Coal with Colby

coal is formed through 300 million years of heat and pressure

starts off as plant life

natural gas is a big part of making coal

types of coal :

Peat- plant life that is decomposing by heat and pressure

Lignite- is peat but is subject to more heat and pressure has dark brown color and crumbles easily  

Subbituminous and Bituminous - basically the same thing subject to less heat and pressure

           anthracite

anthracite- rarest to make about 350 million years to make most efficient to all other coals







Natural Gas with Scott







Oil with Nick
Oil is a fossil fuels meaning that over millions of years, dead plants, and animals turned into oil
Fossil fuel-
Majority of plants and animals that for oil are tiny zooplankton and algae
Oil is collected by drills both on land and off land. Large drills go down to depth right above the oil
Oil is found in rubber, aspirin,crayons, ink
How much oil does U.S collect? 9.4 million barrels
Oil is a pollutant
Oil spills in the ocean can be devastating

3 vocab-
Diatom- plankton most often used in the formation oil
Barrels- 42 U.S. gallons
Fossil fuel- A fuel that has been produced from plant and animal matter


Environmental Impacts with Audrey
Carbon can last 100-300 years
leads to smog acid rain toxins in the environment and air pollution
of co2 comes from power plants
oil- powers mostly everything, cars, planes, houses
when petroleum is burned it emits CO2 = global warming
nitrogen oxide- respiratory
gas- methane, ethane, propane
-main contributor of co2 is cars

3 vocab-
-Air pollution- a mixture of solid particles and gasses in the air that can contaminate atmosphere  
-Global warming- term used to describe a gradual increase in the average temp of the earth atmosphere and oceans, changing earths climate
-Emission (CO2)- production of discharge of something especially gas and radiation


Station 2- Alternative Energy with Kelly
Formed over millions of years
Intense pressure high heat and time
Renewable source is a substance that can be replenished just as fast as it is being drawn out
Fossil fuels not renewable
Cannot be replenished
We really use the equivalent to 4 billions ton of oil
By 2088 we are expected to run out of most fossil fuels



Station 3- Political Ramifications with Casey

Stop global warming by stop using fossil fuels
No perfect energy source

3 vocab-
fossil fuels-A fuel that has been produced from plant and animal matter
Export- no longer needed to import
Energy dependence-

Deep dive 




Crash course is a credible youtube channel because it is very famous resource that many people use around the world. This channel was mean't to help high school students as well.

From this video I have learned what the electron transport chain is and overall cellular respiration.

As a learner I believe this video has helped a lot, it is meant for young people and shows very good visuals helping me understand what it means. 

Ecology #3 



Word
Part of speech
Definition
Fauna
Noun
The animal life in an area
Flora
Noun
Plant life in an area
Food web
Noun
Many food chains put together to show how energy flows through ecosystem
Food chain
Noun
The order that animals feed of of like plants and animals
Population
Noun
All members one species in one area
Predator
Noun
Animal that hunts, kills and eats
Prey
noun
An animal hunted, eaten and killed by predator
Producer
Noun
An organism that makes own food
Primary producer
Noun
Animals eat primary consumers- plant eaters
Secondary consumer
Noun
Animals that eat primary consumers- carnivores and herbivores
Tertiary consumer
Noun
Carnivore top of food chain feeds other carnivores or only secondary

Fossil Fuels

Group of energy sources that were formed from different plants and organisms during the Carboniferous Period around 360 million years ago


Different types of fossil fuels depend on four things the organic matter, temperature, time, and pressure conditions while decomposing

3 Major Types: coal, oil, and natural gas
coal- plants and trees + pressure and heat that hardened 
oil- zooplankton and algae pressure caused to decompose + more heat and pressure

fossil fuels are sought after energy sources because they have a high energy density
world's dominant energy source
considered non-renewable resources because they take millions of years to form
cant be re-used
carbon dioxide causes environmental and health issues
Fossil fuels are largest emitters of carbon dioxide

Ecology vocab #2



Word
Part of speech
Definition
Decomposer
Noun
Organism such as bacteria or fungus that breaks down dead matter and returns nutrients to the soil
Estuary
Noun
A body of water where freshwater and saltwater ecosystems merge together (marine and freshwater ecosystems will be present here)
Habitat
Noun
The place and surroundings where an organism normally lives
Herbivores
Noun
An organism that eats only plants
Host
Noun
A living organism on which a parasite lives
Limiting factor
Noun
Something in an environment that keeps the population
Niche
Noun
The habitat that supplies everything needed for a species or an organism to survive
Omnivores
Noun
A consumer that eats both plants and meats
Parasite
Noun
An organism that lives in or one another organism (called host)

A pond of bacteria? 

Notes: 
pond is a pool of water and sun can penetrate to the bottom 
Ponds are tangent No movement Organisms’ outputs remain in water
Enter bacteria natural decomposer, Breaks down waste and ammonia
reduces algae

3 target vocab: 
Macroscopic- large enough to be viewed without a microscope
Photoautotrophs- organisms producing their own food via photosynthesis
Heterotrophs-organisms that cannot produce their own food


Chlorine and lead in top water 

Notes :
Tap water comes from rivers, sewer, ponds and ground 
2% potable 
Chlorine- 
toxic, irritant, pale green gas, kills bacteria microbiological organisms 
Chlorine affects environment reacts with other chemicals, low level harm 
Lead- 
color, NEVER safe, lead piping, soft or corrosive 
lead affects air pollution,soil, moves through ecosystem, organ damage 

3 target vocab:
corrosivecauses corrosion
potable- safe to drink
microbiological- science that deals with microorganisms.


pH and the Ocean 

Notes:
pH is the acidity in a solution 
8.0-8.4 
Low number- acidic 
High number- alkaline 
Environment can affect pH levels 
pH indicators are weak acids or bases, change specific color at specific pHs 
Color change occurs when acid or base accepts or donates protons 
Past 300 million years has been 8.2 now its 8.1

3 target vocab: 
Acid- molecule that can donate a proton or accept electron pair in reactions 
Bases- molecule that accepts protons 
proton- particle found in nucleus with a positive charge 



Water cycle demo 

Materials: 
-flask with hot water (500 mL water)
-6 mL of morton salt 
-1,000 mL beaker 
-25 mL beaker 
-few ice cubes 

Procedures: 
1. added 6 mL of Morton salt in water 
2. small beaker inside big beaker 
3. add bb's in order to weigh down small beaker (standing up) in big beaker 
4. add plastic wrap to act as the atmosphere 
5. Add ice cubes on top of plastic wrap 
The photo up above is showing the type of salt we used to make the water "salt water".
This photo is showing the flask we used to hold the hot water. (photo above)
this photo is showing our teacher putting the plastic rap over the beakers to keep everything locked in. (photo above)
In person you would be able to see the water droplets on the plastic.

Ecology- Water cycle 


 "Biogeochemical cycles: Figure 1" by OpenStax College, Concepts of Biology,

(above) The image above is showing the saltwater and freshwater there is. As you can see fresh water is very limited. most of the fresh water is iced and not melting, If it melts it will add to the amount of fresh water we have. This graph is for the oceans worldwide. 

"Biogeochemical cycles: Figure 3"  by OpenStax College, Biology

(above) This image is showing where the water is in specific areas.
                         

 The water cycle  by NOAA National Weather Service Jetstream,

(above) the image above shows the cycle in which the water goes. Starting from the a body of water and ending in a body of water.

Ecology Vocabulary 

Word
Part of speech
Definition
Ecology
Noun
The study of how living things interact with one another
Ecosystem
Noun
All living and nonliving things in an area and their interactions
Abiotic factor
Noun
The part of the ecosystem that is not alive and has never been alive
Biotic factor
Noun
The part of an ecosystem that is alive
Adaptation
Noun
A characteristic that helps an organism survive in its environment
Biome
Noun
A plant and animal community that covers a large part of the earth
Detritivore
Noun
An organism (as an earthworm or a fungus) that feeds on dead and decomposing organic matter
Community
Noun
A group of organisms living together in a certain area
Consumers
Noun
An organism that survives by eating producers or other consumers in its ecosystem
Carnivore
Noun
An organism that eats only other consumers (meat eater)
Deforestation
Verb
The cutting down and clearing of forest land- will usually lead to increased soil erosion in this area

Intro to Ecology Demo





Vocabulary:
Abiotic factor- non living things that affect living organisms (soil, water, sun)
biotic factor- living things (frog)
predator- one that preys (bears. wolves)
prey-  taken by predator as food
consumer- organisms (animals) that feed off of other plants and animals
omnivore- feed off of both animal and plant
herbivore- feed off of plants
carnivore-feed off of meat/ animals
autotroph- organism capable of getting its own food (plans <-- sunlight )
photoautotroph- organism gets energy from light and uses carbon as its source
decomposer- helps organic material to break down/decompose
producer- organic compounds using light energy, producers in food chain (algae in water)
primary consumer- herbivores
secondary consumer- carnivores
tertiary consumer-carnivore

Activity:    In this ecology demo we were put in a group of 10-12 students an each given a card with a biotic or abiotic factor. We were told to make our food chain and connect each organism to each other in someway. Then we labeled our abiotic or biotic factor on what it is using the vocabulary up above. The theme of our group was by a river considering we had birds, bacteria, fish, snakes and so on. Through out this lab we documented our progress

 

Science- Flipped classroom videos 

Intro to DNA
Where is DNA found?
   Cell- nucleus- chromosome (DNA) 
What has DNA? 
  Anything that is living has DNA (humans, flowers,) 
What is DNA?
   A molecule, molecules make up organelles 
Macromolecules
  carbohydrates, lipids, nucleic acids, protein 
Structure of DNA
  ladder format 
The function of DNA
  gene coding (eye color, hair color) 
  codes for proteins (amino acids) 
  DNA replication (reproduction, growth skin when cut) 
Fun facts!
  • Over 99% of our DNA sequence is the same as other humans'
  • If you unwrap all the DNA you have in your cells you could reach the moon 6,000 times 

Who killed Romeo and Juliet


                            Suicide mistaken as homicide

    Introduction: “Fathers are more likely to kill. Men killed six out 10 children”  (Bello,         USA Today) While investigating the ‘suicides’ DNA was found at Romeo and Juliet's      crime scene leading the FBI thinking it is Capulet, Juliet's father leaving behind his          DNA. Capulet abominated Romeo knowing he was in love with her daughter and did n   ot want her to marry him, Capulet found out they secretly got married he was                      indignant and he wanted to do something about it. Juliet's father then murdered                  Romeo, Juliet was already awake from her deep sleep and caught his father doing it    ,in order to keep her quiet he had to murder her too, he had set up the crime scene              to make it look like a double suicide.

    Purpose: Whose DNA will match the DNA at Romeo and Juliet's crime scene?  

  Hypothesis: I believe this is true because he wanted Juliet to marry Paris but she              wanted to marry Romeo. Capulet hated Romeo and so Capulet murdered him and his    own daughter because he despised her.

If Capulet killed Romeo and Juliet then he would be the reason of their death because          he did not want Romeo and Juliet to get married he wanted Juliet to marry Paris he         then killed his own daughter because he was furious with her.

    Materials:
  • 2 teaspoons (10 ml) 0.9 percent salt water (2 teaspoons table salt in one quart/liter of water)
  • 4 disposable plastic cups
  • Plastic travel soap box
  • Stainless steel wire, the gauge should be no larger than 18 and no smaller than 24
  • Wire cutters
  • 9-volt batteries (5)
  • Alligator clip leads (2)
  • Styrofoam tray or flat piece
  • Scissors
  • 4 large test tubes
  • 1 teaspoon (5 ml) 25 percent mild detergent or dishwashing soap (1 volume detergent or soap + 3 volumes water)
  • 2 teaspoons (10 ml) 95 percent ethanol, chilled on ice
  • small test tube  (1 for each DNA sample you will collect)
  • 5 ÎĽl methylene blue solution for each DNA sample (Neto pre-made 1 L of water to 2ml of methylene blue)
  • Micro pipette and 4 disposable pipette tips
  • Baking soda
  • Deionized water
  • Glycerin
  • Cold Ethanol 95%
  • Knife



      Procedure:
Making the Gel Electrolysis Chamber


  1. Begin by making the gel electrolysis chamber. Cut two pieces of the stainless steel wire with your wire cutters. The wire should be slightly longer than the width of the plastic box.
  2. Bend the wires so that they hook over the sides of the plastic box and run the width of the box. Place one wire at the top of the box; this will be your negative electrode. Place the other wire at the bottom of your box; this will be your positive electrode.
  3. Using a pair of scissors, cut out a comb out of the Styrofoam with four teeth, one for the murderer and three for the suspects.The comb will be placed vertically into the plastic box and need to stand upright, so it should be wider at the top so that the comb can rest on the edges of the plastic box. The teeth should be evenly spaced and there should be at least 2 millimeters of space between the bottom of the teeth and the bottom of the plastic box.
IMG_2827.JPG
  1. Make the buffer solution that you will use for both making the agarose gel and running the samples. The buffer should be a 1% solution of baking soda. So for every milliliter of water, add one gram of baking soda. The volume of the box was 500 millimeters so 5 grams of baking soda were added to the buffer solution.
  2. Make a 1% agarose gel solution by combining 1 g of agar powder with 100 mL of your buffer solution in a microwave-safe bowl. (If you don't have a kitchen scale, 1 g of agar is approximately ¼ teaspoon.)
    1. Heat the agarose solution in a microwave to dissolve the powder. Stop the microwave every 10-15 seconds to stir the solution.
    2. When you see that the solution is starting to bubble, remove it from the microwave. The solution should be translucent. Make sure to watch the agar solution carefully and remove it promptly from the microwave; when it gets hot it will easily bubble over.

Collecting the DNA
  1. Murder suspect will complete this procedure.  Swish 2 teaspoons (10 ml) 0.9 percent salt water in your mouth for 30 seconds. This amount of swishing will actually become quite laborious
  2. Spit the water into your cup.


Extracting the DNA
  1. Make your soap and water solution (enough for all your DNA samples) Using a large test tube containing 5 ml of liquid detergent to 15 ml of water (25% mild detergent)
  2. Take 5 ml of each sample of DNA that you plan on collecting and place them in their own test tube. Be sure to label each test tube carefully.
  3. Add 5 ml of your mild detergent to each test tube.
  4. Cap tube and gently rock it on its side for 2-3 minutes. The detergent will break open the cell membrane to release the DNA into the soap solution. Do not be too vigorous while mixing! DNA is a very long molecule. Physical abuse can break it into smaller fragments, a process known as shearing.
  5. Open and slightly tilt the tube and pour 1 teaspoon (5 ml) of the chilled 95 percent ethanol down the side of the tube so that it forms a layer on the top of your soapy solution.
  6. Allow tube to stand for 1 minute.
  7. Pull 0.25 ml DNA from the top of the test tube into a micro test tube. Your DNA should stay solid in this solution.
  8. Using the disposable pipettes , add 0.25 ml methylene blue solution for each DNA sample
  1. Add one drop of glycerin to DNA/methylene blue solution. (Be sure all micro tubes are labeled clearly)
Adding the DNA to the Gel Chamber

  1. Make new buffer solution (The buffer should be a 1% solution of baking soda. To make this, combine 2 grams (g) of baking soda with 200 mL of bottled water in one of your bowls and stir well. (If you don't have a kitchen scale, 2 g of baking soda is approximately ½ teaspoon.)
  1. Pour your buffer solution over the solidified gel. Add enough buffer to submerge the gel.
  2. Gently pull the comb out of the gel. Be sure not to remove the comb until you are sure that the agarose gel is completely set. The resulting wells will be used as reservoirs for your samples.
  3. Using the butter knife, carefully cut a thin slice of the gel from the top and the bottom to make room for the electrodes.
  4. Re-attach the stainless steel wire electrodes.
  5. Using a plastic syringe or medicine dropper, fill each well in the gel with each suspects DNA solution
  6. Using the alligator clip leads, attach the battery pack to the wires resting on the gel chamber. The positive terminal of the battery pack should be connected to to the clip farthest away from the DNA samples.
  7. Before turning on your electricity double check with Neto. You will be setting machine to 150 Volts. Be sure not to to touch or disturb the box in the process.
  8. You should see bubbles forming around the electrodes in the buffer as the current passes through them.
    1. If you don't see bubbles, recheck all your electrical connections. Make sure the batteries are properly placed in series, and that the batteries are fresh and fully charged.
  9. Turn off electricity and clean-up lab.



Data Table:
 
    Suspect
Distance the DNA went in the gel chamber
(mm)
   Murderer
Not conclusive
   Capulet
Not conclusive
   Friar John
Not conclusive
   Friar Laurence
Not conclusive


Identify the Independent and Dependent Variable:

  • X: Person’s DNA
  • Y: How far the DNA travels
Graph:



    Conclusion:
   The real murderer was Friar Laurence this infers that his DNA was more identical to the murders DNA.    The gel chamber would have shown the matching of the DNAs if the lab was definite. There was not         pattern in data because the lab did not work how we wanted it to.
 
   My groups’ original hypothesis was wrong, thinking Capulet was the murderer.There were many errors in this lab, instead of using stainless steel wires the wire we used was not stainless steel causing it to rust in our gel chamber. The combs in our gel chamber also pulled out the gel leaving a rectangle space and so we had to cut out our own slits. Our class also did not use enzymes to break up the DNA which would have made it easier. Our lab was definitely not conclusive we had many errors and we only based this on off of one trial because all the materials used were very expensive. The DNA in the gel chambers were really hard to see, my group was not able to see the ending product. If someone else followed          these procedure they might want to change some things like having stainless steel wire, and making       sure not to leave the gel to dry for too long which caused our Styrofoam to pull out the gel and do this      lab in a more suitable environment.


Reminders

System or order = Human body, organ system, organs, tissue, cells, organelles, molecules, atoms  
All enzyme names end with ase - Protase = enzyme used to extract DNA

The Brain

FUN FACTS
Brain is fattest organ
Humans continue to make new neurons
Children who learn 2 languages before the age of five alter the brain structure
CONT..
Word brain appears 66 times in the play in shakespeare  

Quick system of order review
All humans are made up of organ systems
All organ systems are made up of organs
All organs are made up of tissues
All tissues are made up of cells
All cells are made up of organelles
All organelles are made up of molecules
All molecules are made up of atom

Dendrites: brings info to cell body
Axons: Takes info. away from the cell
myelin: helps speed impulse made up of fat and protein
synapse: space between neurons where molecules are transformed


The anatomy of the brain


    The day I woke up in the hospital I was extremely disoriented and confused, then the doctor had just explained to me I had a stroke. This was not what I expected or wanted to hear, the fact that I had just had a stroke was very unexpected, and left me and my family completely shocked and concerned. My doctor had mentioned that my stroke had occurred because of the lack of blood flow in my brain and my brain was not receiving enough nutrients or oxygen. The side of the brain affects the opposite side of the body, in this case my right brain was the area of the stroke and my left side of my body was affected,  I have many challenges to go through since this stroke has happened. This  had caused physical and psychological damage.

    The stroke affected the left side of my body, I ignore the objects and people on my left side and lost movement in my left arm and leg. Walking, eating, getting ready are all affected by my stroke and loosing movement in my arm and leg making it more difficult to complete these tasks. My vision is not as good as it should be on my left side, I may sometimes not recognize shapes or familiar faces, I know this may hurt my family a little but they do understand why I may not be able to recognize them. My doctor had mentioned to me that I should look around often when I am walking around to be aware of where I am walking and to make sure I’m not running into things.

    Along with the physical damage I had psychological damage on right side of my brain. I have trouble keeping up and understanding a conversation I may be having. I can forget what I have said when answering questions and may repeat an answer or phrase. It may be frustrating with other people when I can not hold up a conversation. My doctor had mentioned problem solving and reasoning can affect me.

Even though my stroke will not be totally recovered I will attend a rehabilitation center, I will have to learn how to be without the use of my arm and leg to make some tasks a bit easier. This is not something I expected  but will have to live with what happened. I am now home and i hope to not return to the hospital.   





Word
Function
Brainstem (September)
Controls of flow of actions from the brain to the rest of the body
Cerebellum (September)
Receives information from sensory systems
Cerebrum (Makenzie)
It is the largest part of human brain and it is divided into four parts.
Right brain (Makenzie)
The right brain coordinates the left side of the body and creates creativity and arts.
Frontal Lobe (Jordan)
Controls cognitive skills like emotional expression, problem solving, and memory
Sensory Strip(Jordan)
A band of neurons in your cerebrum or cerebral cortex that controls the 5 senses.
Medulla(Riley)
The medulla oblongata helps regulate breathing, heart and blood vessel function, digestion, sneezing, and swallowing. This part of the brain is a center for respiration and circulation. Sensory and motor neurons (nerve cells) from the forebrain and midbrain travel through the medulla.
Pons (Riley)
The part of the brainstem that links the medulla oblongata and the thalamus.
Occipital (Alyssiana Hugel)
One of the four major lobes, that is responsible for your vision.
Meninges(Alyssiana Hugel)
Three membranes that cover the spinal cord and the brain. The three membranes are dura mater, arachnoid mater, and pia mater.
Thalamus(Will)
The thalamus is responsible for the sleep and wake cycle
Temporal(Will)
The temporal lobe is mostly responsible for hearing
Parietal  (Lyric)
attached to the wall of the body or of a body cavity or hollow structure.



Hypothalamus (josh)
a region of the forebrain below the thalamus that coordinates both the autonomic nervous system and the activity of the pituitary, controlling body temperature, thirst, hunger, and other homeostatic systems, and involved in sleep and emotional activity.
Pituitary gland (josh)
Controls hormones in the body and triggers puberty in the body so that it may grow to it’s full adult form
Pineal gland (erika)
Small endocrine gland in the vertebrate brain. The pineal gland produces melatonin, a serotonin derived hormone which modulates sleep patterns in both circadian and seasonal cycles.
Broca’s Area (erika)
region in the frontal lobe of the left hemisphere of the brain with functions linked to speech production.

Iron chef lab presentations

Pesticides

Team 1
Team 2
Hypothesis
Affect more animal cells because affects nervous system

If organic and inorganic samples of: ground chuck and apples are exposed to the same pesticides than the organic foods cellular structure will be more damaged by the pesticides then the non organic foods because non organic food has been altered to resist pesticides.
Skills or techniques
Yeast
Made agar
Results of lab
Correct hypothesis
More growth on organic
Do differently/ errors
Ants
Turning off incubator
Smelt bad
length/time not exact
Incubator overheated
Have more order, controlled environment, more measurements
Questions and/ or comments



Sugars

Team 1
Team 2
Hypothesis
If bacteria is exposed to various different sugars
If six students, three boys, three girls get their glucose levels tested for one week
Skills or techniques
Making agar
pricking , Researched best times to test
Results of lab
Honey highest growing of bacteria hypothesis was wrong
Holes in data, kids absent, kids not meeting at the same time
Do differently/ errors
Incubator turned off
Get more test subjects
Questions and/ or comments




Corn

Team 1
Team 2
Hypothesis
If a plastic water bottle is melted down
If 50g of frozen, canned, and a conventional ear of corn are stored within an incubator at 33° C, than the conventional ear of corn will generate the most bacteria within 6 observational days because no methods have been applied to prevent the growth of bacteria and promote the preservation of the corn.

Skills or techniques
BPA, take
pH levels
Staining
Microscope
Agar
Colony counting
Results of lab
Did not get exact thing looking for because microscope and lack of info on BPA on the internet
Ear of corn grew more bacteria
Do differently/ errors
Not being able to make BPA had to use
Turned off incubator
Questions and/ or comments




Drinks

Team 1
Team 2
Hypothesis
If 15 human teeth were
If yeast is given different liquids with varying amounts of natural and artificial sugars in bottles with balloons on top, then the bottle with the most natural sugar will inflate the balloon the most because the yeast will thrive with the natural sugars causing the yeast to release more carbon dioxide.

Skills or techniques
Measuring grams from teeth weight
Agar
Yeast carbon dioxide & balloons
Results of lab
Coca cola had the biggest impact on teeth
Honey had most impact but then cane sugar was most inconsistent
Do differently/ errors
Incubator turned off
Heated incubator
Drinks spilled (brought ants)
Sugar and honey overflowed
Ran out of certain ingredients
Questions and/ or comments



Team 2

 Iron chef lab - 

 In this lab we students had a mystery box my group ended up with strawberries. Five other group members and I made up a lab using strawberries and we are competing with the other groups to have the best lab. We decided to do something with mold since we already knew mold grew fast on strawberries. Our lab was to identify botrytis mold on organic and conventional strawberries and on which one grew more mold. We used agar and patato dextrin agar to find out which one produced that grey mold.

Procedures:
  1. Make Regular Agar and pour into 6 petri dishes.
  2. Make PDA and pour into 6 different petri dishes
  3. Refrigerate for 3 days
  4. Swab the outside of organic strawberries and place into 2 PDA dishes and 2 RA dishes
  5. Swab the outside of conventional strawberries and place into 2 PDA and 2 RA dishes
  6. Leave 2 RA dishes and 2 PDA dishes alone as a control
  7. Put all 12 dishes into an incubator
  8. After 1 week of incubation Take out 1 dish from each group (conventional pda, control pda, organic pda, conventional ra, control ra, organic ra) and use the ocular loop to scrape off a sample from each dish.
  9. Transfer these samples to individual slides and cover them with slide covers.
  10. Place the slides under microscopes and examine them for botrytis cinerea.
  11. If it is a positive id then mark it as such in the data table.
  12. Repeat steps 7-10 at  1.5  weeks for the other set of petri dish samples.
  13. Repeat steps 7-10 again at 2 weeks for the first set of petri dishes.
  14. Repeat steps 7-10 again at 2.5 weeks for the second set of petri dishes.
Materials:
  • 1 box of strawberries (organic)
  • 1 box of strawberries (non organic)
  • Potato Dextrose agar
  • Basic Agar
  • Petri dishes
  • Microscope
  • Slides
  • bvSlide covers
  • Swab
  • Iodine  (staining as needed)
  • Incubator
  • Ocular Loop
Skills

  • Fungus identification
  • Microscope usage
  • Incubator usage
  • Agar plate making

Hypothesis-
If a lab technician swabs a organic and a conventional strawberry and transfers it into in an agar plate and then an incubator then the organic strawberries’ agar plate will grow more botrytis cinerea than the agar plate with the swab from the conventional strawberry because the organic strawberries are not allowed to use pesticides to prevent mold.   




Making agar
This was two days after we swabbed the strawberry and you could already see the change. ( Regular Agar conventional strawberry)



Cellular Respiration-

The main point of cellular respiration is to take sugars and oxygen turn into adenosine (ATP)

There are three steps to the cellular respiration. The steps occur in the cytoplasm which is the liquid surrounding the cell and the mitochondria also known as the powerhouse. The first step is glycolysis the main point of this step is to break down the sugars. Glycolysis occurs in the cytoplasm this process takes 2 ATP's to breakdown 6 carbon rings it then makes 4 ATP's and pyruvate is a necessary input for the kreb cycle which is then transferred into the mitochondria


Digestive system teach back


Liver
Main functions?
Detoxify blood
Toxins separated
Filters Nutrients, fat stores
Produces bile

Located?
Between pancreas and gallbladder

Liver is dense with many cells that filter and make bile from toxins

Main problems of heart diseases- liver is full of fat, clogged in all arteries
Level of sugar remains constant

Glycogen - takes sugar from the food stores it into that enzyme so when low in blood it is used

Facts
Storage, production metabolizing and filter
Filters into heart
Alcohol leads to liver inflammation.
Gallbladder
Located and size
under the liver
Muscle sack
Size of small pear
7-10 inches long

What is bile?
bile - light green made out of bile salts water and cholesterol
Bile received from bladder squeezes bile into small intest when digested food
Helps enzyme break down helps lipase break the stuff down
Helps in the small intestine
Helps enzyme break down
Gallstone doesn't let the bile out
You can live without a gallbladder
Pancreas
Main function?
Pancreas is one way No food travels through it
Delivers stuff into body
Creates enzymes into the small intestine Enzymes digest food chemically

2nd main function- creates hormones insulin,  Glucagon
Diabetes don’t produce much insulin


Making poop 9/13/16




                  How does food go through our digestion system and end up as poop?

Food digestion first starts off with our mouth and our ingestion of food. As the food enters your mouth it is your incisors, molars, saliva and amylase job to break down the food. Amylase is an enzyme that breaks down starch and glycogen into sugars. The act of the mouths job is called a mechanical digestion; the act of physically breaking down food. From the mouth it goes through the esophagus, it is a tube that connects the throat and stomach. As soon as the food enters the stomach it releases acids and more enzymes and begins the process of churning to break down the food even more. The process in the stomach with the enzymes and acids is called a chemical digestion. The enzymes in the stomach are pepsin and lipase. Pepsin breaks down the proteins as for the lipase breaks down the fat. The stomach also includes hydrochloric acid, this acid protects the body from receiving any diseases. The stomach then leads the food into the small intestine. The small intestine has the higher percentage of most work in the digestive system, this intestine absorbs the nutrition. The large intestine is the last step before the waste exits the body. The large intestine absorbs the water from the stool called absorption and forms it so the waste has a shape that can easily be removed from the body. The rectum is storage for the waste before it exits the body. That is how food goes through our digestion system and ends up as poop.   










This picture includes the mouth, gullet (esophagus), stomach, large intestine, small intestine, rectum and anus these all have big roles in the digestive system.  
In the 'Poop Lab' we did in class each person in this picture was in charge of a step in the digestion system from left to right we start off with the mouth, esophagus, stomach, small intestine, large intestine and ending on the right is the rectum.
The bowl is acting as the mouth holding the food the scissors are acting as the incisors and the two plastic cups were the molars. In this part of digestion it is breaking down into small pieces these students also added water to act as saliva during this process.
The paper rowel roll made from cardboard is playing the role of the esophagus and leading the food into the stomach to help the food digest more.
The stomach is the ziploc bag keeping all the food and acids inside. The girl on the right is putting in liquids to act as enzymes. The stomach is a muscular organ.
These are the ones that are getting poured into the stomach to help digest more.
This nylon material was the small intestine the material fit as this intestine because it absorbs the nutrients and the calories. The small intestine connects the stomach to the large intestine.
The white sock was the large intestine at the end of the sock was a hole acting as the rectum.









                                            


              
                                 

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